Abcam, ab55474, Anti-NBR1 antibody [5C3]

Size: 50µg
Mouse Monoclonal NBR1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Recombinant fragment, Mouse, Human, Recombinant fragment - Human samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NBR1 aa 1-100.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5C3,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:WB, Flow Cyt, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NBR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q14596

Product details:
This product was changed from ascites to tissue culture supernatant on 8/Oct/2019. Lot numbers higher than GR3274169 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NBR1 also known as Neighbor of BRCA1 Gene 1 functions as a receptor for selective autophagy. It recognizes ubiquitinated proteins and delivers them to autophagosomes for degradation. The protein has a molecular weight of approximately 110 kDa. Scientists have observed its expression in a variety of tissues with particularly high levels in the testis and low levels in the lung and kidney.
Biological function summary
NBR1 plays a significant role in the removal of misfolded or damaged proteins through autophagy. It acts within the p62/SQSTM1 complex which links to the autophagic machinery. The complex recruits ubiquitinated substrates facilitating their degradation in lysosomes. Through this mechanism NBR1 helps maintain cellular homeostasis and genome integrity by removing potentially harmful elements.
Pathways
NBR1 integrates into the critical cellular recycling process known as autophagy. It works closely with proteins such as p62/SQSTM1 in the ubiquitin-proteasome pathway to ensure efficient protein turnover. The protein participates in signals that control cell growth and survival influencing the mTOR pathway which is important in regulating cellular metabolism growth and life span.
Research indicates that NBR1 might have implications in neurodegenerative diseases like Alzheimer's and Parkinson's. Its involvement in autophagy suggests that malfunction or aberrant expression of NBR1 could lead to the accumulation of protein aggregates associated with these conditions. Additionally interactions with proteins such as LC3 and p62/SQSTM1 central in lysosomal degradation highlight its potential role in disease mechanisms where autophagy is disrupted.