Abcam, ab55735, Anti-DMT1 antibody [4C6]

Size: 100µg
Anti-DMT1 antibody [4C6] (ab55735) is a mouse monoclonal antibody detecting DMT1 in Western Blot, Flow Cytometry, IHC-P, IHC-Fr . Suitable for Human, Mouse, . - KO validated for confirmed specificity - Over 30 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4C6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:IHC-Fr, Flow Cyt, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SLC11A2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P49281

Product details:
What is this antibody validated in?
Anti-DMT1 antibody [4C6] (ab55735) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, samples.
What is the molecular weight of DMT1?
Anti-DMT1 [4C6] (ab55735) specifically detects a band for DMT1 (UniProt: P49281) at a molecular weight of 62kDa.
Trusted by the scientific community
Anti-DMT1 [4C6] (ab55735) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-DMT1 antibody [4C6] (ab55735) has been confirmed by Western blot testing in SLC11A2 Knockout A549 cells.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: 100% 1X PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DMT1 protein also known as divalent metal transporter 1 or NRAMP2 functions as an essential transporter for divalent metal ions including iron and manganese. DMT1 has a molecular mass around 65 kDa and is expressed widely in tissues like the intestine liver and brain where it facilitates the uptake of these ions. In the gut it plays a significant role in iron absorption from dietary sources. DMT1 protein contributes to maintaining systemic iron levels important for many physiological processes.
Biological function summary
DMT1 facilitates cellular uptake of iron important for oxygen transport and DNA synthesis. As part of its function DMT1 operates independently not as a component of a larger protein complex enabling it to transport metal ions across cell membranes directly. This process ensures that cells receive necessary ions for their metabolic activities and energy production aiding in cellular homeostasis.
Pathways
DMT1 integrates into important biological systems like the iron metabolism pathway and the manganese transport pathway. It works alongside ferritin a protein responsible for storing iron within cells to regulate iron availability and prevent toxicity. Similarly its role in the manganese transport pathway helps maintain manganese homeostasis which protease enzymes and neurotransmitter synthesis rely on.
DMT1 shows significant relevance to iron overload conditions such as hemochromatosis and neurodegenerative diseases like Parkinson’s disease. Mutations or dysregulation in DMT1 expression can lead to improper iron accumulation contributing to oxidative stress and neuronal damage respectively. Its role in iron uptake and regulation therefore connects it with proteins like hepcidin influential in maintaining iron balance impacting these conditions.