Abcam, ab55811, Anti-C5b-9 antibody

Size: 100µg
Anti-C5b-9 antibody (ab55811) is a rabbit polyclonal antibody detecting C5b-9 in IHC-P, ICC/IF . Suitable for Human . - Over 80 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human C5.P01031,
Specificity:This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma

Product details:
Anti-C5b-9 antibody (ab55811) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P.
Anti-C5b-9 antibody (ab55811) has been cited over 82 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-C5b-9 antibody (ab55811) has high sensitivity and specificity.
Anti-C5b-9 antibody (ab55811) has 4 independent reviews from customers.
Anti-C5b-9 antibody (ab55811) specifically detects C5b-9 (UniProt ID: P13671; Molecular weight: 102kDa) and is sold in 100 µg selling sizes. The C5b-9 complement complex is a membrane attack complex that's part of the body's innate and acquired immune system and is formed when the complement system is activated.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-This antibody is provided as IgG fraction. Please note that the concentration value refers to the fraction., Storage buffer-pH: 7.2Preservative: 0.1% Sodium azideConstituents: 99.9% PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C5b-9 also known as the membrane attack complex (MAC) serves as a critical component in the immune system's defense mechanism. Composed of proteins C5b C6 C7 C8 and multiple units of C9 this complex forms on the surface of pathogen membranes. It typically weighs approximately 900 kDa. The MAC largely expresses on cell membranes during immune responses and primarily targets bacterial pathogens by creating pores that lead to cell lysis.
Biological function summary
C5b-9 functions as part of the larger complement system which aids in innate immunity. The process begins once C5b initiates the assembly of the complex by binding to C6 C7 C8 and finally incorporating C9 molecules. These form a pore-like structure leading to increased permeability of the target cell membrane resulting in the disruption and elimination of the pathogen.
Pathways
C5b-9 plays a critical role in the complement cascade an essential pathway in the immune system. This cascade consists of several steps including the alternative and classical pathways. C5b-9 is related to the protein C3 through these pathways which upon activation helps facilitate C5 activation. This connection highlights its importance in promoting pathogen clearance.
C5b-9 has associations with conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH excessive formation of this complex on red blood cells leads to their destruction contributing to hemolysis. Similarly in aHUS dysregulation of the complement system including C5b-9 causes damage to endothelial cells. This complex connects with proteins like factor H and CD59 whose dysfunction or deficiency can lead to or exacerbate these diseases.