Abcam, ab56800, Anti-SF3B2 antibody [5D2]

Size: 100µg
Mouse Monoclonal SF3B2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SF3B2 aa 550-650.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5D2,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SF3B2 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.Q13435

Product details:
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.4, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SF3B2 protein also known as SAP145 is a component of the spliceosome complex important for RNA splicing. It has a significant molecular weight of approximately 145 kDa. SF3B2 expresses in various tissues throughout the human body indicating its importance in fundamental cellular activities. The protein mainly localizes to the nucleus where it interacts with other splicing factors to regulate the removal of introns from pre-mRNA.
Biological function summary
This protein plays a critical role in the pre-mRNA splicing process acting as part of the larger spliceosomal complex SF3b which is essential for accurate exon recognition and splice site selection. By ensuring the correct processing of genetic information SF3B2 influences gene expression and stability. It participates in the formation of the spliceosome's U2snRNP sub-complex which is necessary for splicing fidelity and overall cellular function.
Pathways
SF3B2 engages primarily in the mRNA splicing pathway which directly impacts gene expression and regulation. It associates with the splicing factor 3b complex which incorporates multiple proteins for efficient splicing. Its interaction with other spliceosomal proteins such as SF3B1 and U2AF facilitates the understanding of the exact splicing mechanism and highlights SF3B2's central role in these pathways.
Altered SF3B2 expression and function connect to cancer and myelodysplastic syndromes with SF3B1 mutations often observed in similar contexts. Researchers link spliceosomal dysfunctions caused by SF3B2 to aberrant splicing events contributing to oncogenesis and hematological disorders. As a result studies investigate SF3B2 as a potential target for therapeutic intervention focusing on restoring normal splicing activity.