Abcam, ab57573, Anti-SMURF1 antibody [1D7]

Size: 100µg
Mouse Monoclonal SMURF1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMURF1 aa 150-300.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1D7,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ICC/IF, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SMURF1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.Q9HCE7

Product details:
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMURF1 also known as SMAD-specific E3 ubiquitin protein ligase 1 functions as an E3 ubiquitin ligase involved in the targeted degradation of proteins. This protein weighs approximately 72 kDa and expresses primarily in various human tissues including bone lung and kidney. SMURF1 plays an essential role in regulating protein turnover by tagging substrates for proteasomal degradation. It interacts with substrates through a C2 domain which aids in membrane localization and substrate recognition effectively controlling cellular protein levels and their functions.
Biological function summary
SMURF1 influences numerous cellular processes such as bone morphogenetic protein (BMP) signaling by regulating the availability and stability of signaling molecules. It does not function in isolation; it is a component of a ubiquitin-proteasome system complex that includes other cofactors assisting its activity. SMURF1 therefore affects cell growth differentiation and homeostasis by managing signal transduction pathways critical for these cellular activities.
Pathways
SMURF1 is involved in the BMP and Wnt signaling pathways. Both are fundamental for various biological activities including embryonic development and cell cycle regulation. Within these pathways SMURF1 influences the activity of SMAD6 and related proteins modulating their turnover and impact on downstream signaling processes. These pathways also interact with other cellular pathways but SMURF1's specific role remains concentrated on modulating protein ubiquitination events.
SMURF1 has been linked to cancer and osteoporosis. Its dysregulation can disrupt normal cellular signaling contributing to tumorigenesis or impaired bone maintenance. In cancer its interaction with proteins such as SMAD7 can alter cell signaling promoting uncontrolled cell growth. In osteoporosis SMURF1's regulation of bone remodeling processes might be compromised leading to decreased bone mass and fragility. Understanding SMURF1's roles may open avenues for therapeutic interventions in these disorders.