Abcam, ab5782, Anti-PKC beta 1 (phospho T642) antibody

Size: 50µL
Rabbit Polyclonal KPCB phospho T642 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCB phospho T642.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKCB phospho T642. The exact immunogen used to generate this antibody is proprietary information.P05771,
Specificity:This antibody does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT710], iota [pT555], eta [pT655], or zeta [pT560] as determined by peptide competition experiments.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC beta 1. The final product is generated by affinity chromatography using a PKC beta 1-derived peptide that is phosphorylated at threonine 642., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PKC beta 1 (Protein Kinase C beta 1) is an enzyme that belongs to the protein kinase C (PKC) family. It plays an important role in signal transduction processes. This protein has a molecular mass of approximately 80 kDa and is known to express in various tissues including the brain heart and spleen. PKC beta 1 undergoes phosphorylation upon activation which alters its structure and function. It localizes to the cytoplasm and also to the cellular membranes under certain conditions.
Biological function summary
PKC beta 1 is involved in regulating various cellular functions such as cell survival differentiation and proliferation. It operates as part of a larger kinase complex and influences downstream targets via its catalytic activity. The activity of PKC beta 1 impacts cytoskeleton reorganization and vesicular trafficking. It interacts with other PKC isoforms and different substrates contributing significantly to cellular homeostasis.
Pathways
PKC beta 1 plays a major role in the MAPK/ERK and PI3K/AKT pathways. These pathways are essential for transmitting signals from growth factors and hormones to cellular responses. PKC protein's interaction within these pathways involves modulation of other proteins like Raf-1 and Akt which are critical for executing biological outcomes. PKC beta 1 through its influence in these pathways partakes in controlling cell fate decisions.
PKC beta 1 has connections to diabetic complications and certain cancers. Its altered expression or dysregulation links with the progression of diabetic retinopathy by impacting vascular endothelial growth factor (VEGF) production. Additionally atypical PKC beta 1 signaling contributes to tumorigenesis in cancers like chronic lymphocytic leukemia. PKC protein’s aberrant activity in these conditions associates with altered survival pathways and resistance to apoptosis.