Abcam, ab5803, Anti-CREB + ICER antibody

Size: 100µg
Rabbit Polyclonal CREB phospho S133 + S133 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human cAMP-responsive element modulator aa 100-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human cAMP-responsive element modulator aa 100-150. The exact immunogen used to generate this antibody is proprietary information.Q03060,
Specificity:ab5803 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cAMP response element-binding (CREB) protein with alternate names such as cAMP-responsive element modulator (CREM) or inducible cAMP early repressor (ICER) functions as a transcription factor that binds to specific DNA sequences called cAMP response elements. CREB is approximately 43 kDa in size and plays a critical role in translating extracellular signals into gene transcription. Expression of CREB occurs in various tissues but is especially notable in the brain and some peripheral tissues like the pancreas. This protein exists in several isoforms contributing to its functional diversity in different cellular contexts.
Biological function summary
The CREB protein family including ICER participates in modulating a wide array of cellular processes especially in relation to the nervous system. CREB functions as part of a transcriptional complex requiring phosphorylation at specific serine residues for activation. The active state of CREB can promote the transcription of numerous genes involved in cell survival proliferation and metabolic processes. ICER an inducible repressor form acts as a regulator within the CREB family providing feedback control by down-regulating CREB-mediated transcription.
Pathways
CREB plays a significant role in the cAMP signaling pathway and the calmodulin-mediated pathway. The cAMP signaling pathway utilizes adenylate cyclase to convert ATP to cAMP activating protein kinase A (PKA) which in turn phosphorylates CREB triggering transcription of downstream genes. CREB also interplays with other proteins like BDNF (brain-derived neurotrophic factor) in pathways governing synaptic plasticity and neuronal survival.
CREB has associations with Alzheimer's disease and depression highlighting its importance in brain health. Alzheimer's disease involves disrupted signaling pathways where CREB-related genes may lose regulation impacting neuronal survival. In depression altered CREB activity might influence mood regulation and response to antidepressants potentially through its interaction with serotonin receptors. Understanding related proteins like BDNF and their relationships with CREB further elucidates its role in these conditions.