Abcam, ab58425, Anti-TIMP4 antibody

Size: 100µg
Rabbit Polyclonal TIMP4 antibody. Suitable for ELISA, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human Metalloproteinase inhibitor 4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:ELISA, IHC-P, IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Metalloproteinase inhibitor 4. The exact immunogen used to generate this antibody is proprietary information.Q99727

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab58425 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tissue Inhibitor of Metalloproteinases 4 (TIMP4) a member of the TIMP family plays a mechanical role by inhibiting matrix metalloproteinases (MMPs). This inhibition helps regulate extracellular matrix (ECM) remodeling. TIMP4 weighs approximately 22 kDa and is expressed in various tissues including the heart brain and reproductive organs. It binds directly to MMPs forming stable complexes that prevent the degradation of ECM components.
Biological function summary
TIMP4 contributes to maintaining tissue homeostasis and integrity. By regulating ECM degradation it influences processes such as cell migration proliferation and angiogenesis. TIMP4 does not work alone; it associates with enzymes like MMP-2 and MMP-14 modulating their activities to keep ECM dynamics in check. This regulation is essential for normal physiological functions and also during tissue repair.
Pathways
TIMP4 participates in the balance between ECM synthesis and degradation. It takes part in the ECM receptor interaction and focal adhesion pathways. These pathways involve several proteins including integrins and collagens which interact with ECM components modulated by TIMP4 activity. TIMP4 helps coordinate cellular responses to environmental changes through these pathways thereby influencing cell behavior and tissue organization.
TIMP4 has associations with cardiovascular diseases and cancer. In cardiac contexts altered TIMP4 levels impact heart function and remodeling often linked to cardiovascular diseases. Its dysregulation can contribute to tumor progression in cancers by affecting ECM degradation and subsequent metastasis. TIMP4's interaction with proteins like MMP-9 links it to both tumor invasiveness and vascular remodeling in these disease states.