Abcam, ab59300, Anti-eIF4B (phospho S422) antibody

Size: 100µg
Rabbit Polyclonal eIF4B phospho S422 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 4B phospho S422 aa 350-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Eukaryotic translation initiation factor 4B phospho S422 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.P23588

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.
Biological function summary
EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.
Pathways
EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.
Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.