Abcam, ab59715, Anti-Clostridium botulinum Toxin B antibody [GR-3G7]

Size: 100µg
Mouse Monoclonal BXB antibody. Carrier free. Suitable for cELISA, Flow Cyt and reacts with Clostridium botulinum, Transfected cell line - Clostridium botulinum samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:GR-3G7,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Clostridium botulinum,
Applications:cELISA, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with Clostridium botulinum Toxin B.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Clostridium botulinum toxin B often called "botulinum toxin B" or "botox B" is a neurotoxic protein produced by the bacterium Clostridium botulinum. This protein has a molecular mass of approximately 150 kDa. It is expressed during the bacterial life cycle and released into environments that promote anaerobic conditions. Mechanically botulinum toxin B interferes with acetylcholine release at the neuromuscular junction. The toxin binds to peripheral neurons enters the cells and cleaves specific proteins required for neurotransmitter exocytosis which results in muscle paralysis.
Biological function summary
The interruption of neurotransmitter release caused by Clostridium botulinum toxin B is essential for its paralytic effect. This action makes it part of a family of botulinum neurotoxins which all inhibit synaptic vesicle fusion through enzymatic cleavage of SNARE proteins. Though it generally acts independently it shares a similar mechanism with botulinum toxin type A another well-known neurotoxin. Through this action botulinum toxin B disrupts normal nervous system communication which can be significant in both clinical and research contexts.
Pathways
Botulinum toxin B is part of the cholinergic signaling pathway where it plays an important role in modulating neurotransmission. Its ability to cleave VAMP (vesicle-associated membrane protein) is important for its inhibitory action on synaptic vesicle fusion. This places it in a similar functional space as botulinum toxin A which targets SNAP-25 another SNARE protein. Both toxins effectively decrease acetylcholine release disrupting normal synaptic activity and muscle contraction processes.
Botulinum toxin B is associated primarily with botulism a serious illness characterized by muscle paralysis that can lead to respiratory failure. However in controlled medical settings it serves as a therapeutic agent in the treatment of dystonia a movement disorder causing muscle contractions and spasms. The therapeutic use relies heavily on its ability to reduce excessive muscle activity by targeting nerves similar to those affected in botulism which involves SNARE proteins like VAMP. This makes botulinum toxins including type A and B integral to understanding both the detrimental and beneficial effects of neuromodulation.