Abcam, ab6046, Anti-beta Tubulin antibody - Loading Control

Size: 100µg / 250µg
Anti-beta Tubulin antibody - Loading Control (ab6046) is a rabbit polyclonal antibody detecting beta Tubulin in Western Blot, IP, IHC-P, ICC/IF . Suitable for Chinese hamster, Human, Mouse, Rat . - Over 1250 publications - Trusted since 2004
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat, Chinese hamster,
Applications:IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects a single clean band at 50kD representing beta Tubulin. This band is significantly reduced by using peptide blocking.

Product details:
Anti-beta Tubulin antibody - Loading Control (ab6046) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, IP and WB.
Anti-beta Tubulin antibody - Loading Control (ab6046) was first used in a scientific publication in 2004 and has been cited over 1253 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta Tubulin antibody - Loading Control (ab6046) has high sensitivity and specificity.
Anti-beta Tubulin antibody - Loading Control (ab6046) has 61 independent reviews from customers.
beta Tubulin antibodies are often used as loading controls in Western Blot. Anti-beta Tubulin antibody - Loading Control has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.
Anti-beta Tubulin antibody - Loading Control (ab6046) specifically detects beta Tubulin (UniProt ID: P07437; Molecular weight: 50kDa) and is sold in 100 µg and 250 µg selling sizes.
Top cited antibody on the market to beta tubulin with >1510 citations. Top rated with >61 reviews. Beta-tubulin is commonly used as a loading control in Western blotting to ensure equal protein loading across samples, as its expression remains consistent under various experimental conditions

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.
Biological function summary
This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell's architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.
Pathways
Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.
Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.