Abcam, ab60721, Anti-FOXA2 antibody [7E6] - BSA and Azide free

Size: 100µg
Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting FOXA2 in Western Blot, Flow Cytometry, IP, ICC/IF . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 20 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:7E6,
Isotype:IgG2a,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, ICC/IF, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FOXA2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y261

Product details:
What is this antibody validated in?
Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of FOXA2?
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) specifically detects a band for FOXA2 (UniProt: Q9Y261) at a molecular weight of 48kDa.
Trusted by the scientific community
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from cell culture supernatant., Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.
Biological function summary
FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.
Pathways
FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.
Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.