Abcam, ab60850, Recombinant human PKC iota protein

Size: 10µg
Recombinant human PKC iota protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>90% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:WB, SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:P41743,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Product details:
ab204856
(CREB peptide) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase C (PKC) iota also known by its gene symbol PRKCI is an atypical member of the PKC family. It has a molecular mass of approximately 74 kDa. PKC iota does not respond to calcium and is insensitive to diacylglycerol and phorbol esters which differentiates it from other classical PKC family members. The protein initiates cell survival and proliferation pathways and is primarily expressed in brain heart liver and skeletal muscle tissues indicating its extensive role across various biological functions.
Biological function summary
PKC iota acts as an important player in regulating cell polarity and protecting cells from stress-induced apoptosis. It forms part of the Par6-Par3-PKC iota complex which is important for maintaining cell polarity and directional migration. This complex is essential for neural tube closure during embryonic development and in establishing cell polarity across diverse cell types. PKC iota also contributes to the regulation of microtubule organization reinforcing its vital role in maintaining cellular architecture.
Pathways
PKC iota is important in the NF-kB signaling pathway and the PI3K/AKT pathway. In the NF-kB pathway PKC iota activates IkB kinase leading to NF-kB transcriptional activity which promotes cell survival. In the PI3K/AKT pathway PKC iota contributes to cell growth and survival working closely with AKT proteins. These pathways highlight its involvement in processes like proliferation survival and cellular response to external stimuli.
PKC iota has a significant connection to cancer and neurological disorders. Its overexpression has been observed in several cancers such as lung and prostate cancer where it is often associated with poor prognosis. PKC iota’s role in the maintenance of cell polarity and survival links it to malignant cell behavior. Additionally in neurological disorders like Alzheimer’s disease alterations in PKC iota activity have been related to synaptic dysfunctions. Connections to proteins such as AKT and NF-kB in these conditions highlight its potential as a therapeutic target.