Abcam, ab61055, Anti-Hck (phospho Y410) antibody

Size: 100µg
Rabbit Polyclonal HCK phospho Y410 antibody. Suitable for ELISA, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human Tyrosine-protein kinase HCK phospho Y410 aa 350-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ICC/IF, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Tyrosine-protein kinase HCK phospho Y410 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.P08631,
Specificity:Detects target only when phosphorylated at tyrosine 410.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hck also known as Hemopoietic cell kinase is a protein with a mass of approximately 59-61 kDa. Hck belongs to the Src family of tyrosine kinases. It is expressed mainly in cells of the myeloid and B-lymphoid lineages such as macrophages neutrophils and certain B-cells. This protein functions as a non-receptor tyrosine kinase which means it's involved in adding phosphate groups to other proteins to modulate their activity. Such phosphorylation events play significant roles in signal transduction impacting cellular responses.
Biological function summary
Hck is involved in immune system regulation and cell signaling processes. It operates within complexes that facilitate its kinase activity and substrate specificity. Hck influences pathways that regulate immune cell responses like phagocytosis and cell migration. It helps in cellular adhesion and the production of reactive oxygen species both of which are vital for the proper functioning of myeloid cells in the immune response.
Pathways
Several signaling processes use Hck for transmitting signals from the outside of the cell to the inside. Hck participates in the Fc gamma receptor-mediated phagocytosis pathway and the chemokine signaling pathway. In these pathways Hck collaborates with proteins such as Src and Lyn enhancing the immune response and cellular communication. These pathways control important immune functions and cellular responses.
Hck has been implicated in certain cancers including myeloid leukemias where its overactivity can lead to uncontrolled cell proliferation. Additionally Hck plays a role in chronic inflammation seen in disorders like rheumatoid arthritis. In these conditions Hck interacts with other proteins such as Fgr and Yes exacerbating inflammation and disease progression. Understanding Hck's function offers insights into potential therapeutic targets for these health issues.