Abcam, ab6160, Anti-Tubulin antibody [YL1/2] - Loading Control

Size: 100µg
Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) is a rat monoclonal antibody detecting Tubulin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 540 publications - Trusted since 2002
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:YL1/2,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:IHC-P, WB, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The YL1/2 monoclonal epitope has been mapped to the last 8 residues (GEEEGEEY) at the carboxy terminus of alpha tubulin when tyrosinated (PubMed IDs: 6415068, 6204858).

Product details:
Product Specifications
Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples.
Tubulin antibodies are often used as loading controls in Western Blot. Anti-Tubulin antibody [YL1/2] - Loading Control has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.
Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) specifically detects Tubulin (UniProt ID: P68363; Molecular weight: 50kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) has high sensitivity and specificity.
Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) has been cited over 545 times in peer reviewed journals and is trusted by the scientific community.
Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) has 36 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone YL1/2 -
ab269872
Antibody clone YL1/2 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, HRP (
ab197737
ab197740
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.
Biological function summary
This core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.
Pathways
Tubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.
Aberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.