Abcam, ab62388, Anti-nNav1.5 antibody [4G8:1G7]

Size: 100µg
Mouse Monoclonal SCN5A antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCN5A.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4G8:1G7,
Isotype:IgM,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SCN5A. The exact immunogen used to generate this antibody is proprietary information.Q14524,
Specificity:The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein nNav1.5 also known as SCN5A functions as a voltage-gated sodium channel often referred to as Naᵥ1.5. It allows the rapid influx of sodium ions into cells aiding in the generation and propagation of action potentials especially within cardiac myocytes. The protein has a molecular mass of approximately 260 kDa. This channel finds high expression in cardiac tissues contributing significantly to the electrical activity of the heart muscle. While nNav1.5 is mostly found in the heart its expression can be detected albeit less prominently in other tissues like the gastrointestinal tract.
Biological function summary
NNav1.5 plays an important role in maintaining normal cardiac rhythm. It is part of a complex that includes various auxiliary proteins such as the beta subunits which modulate its activity and trafficking. nNav1.5's main function involves the initiation and conduction of electrical signals in cardiac cells synchronizing heartbeats. Besides its central role in excitability nNav1.5 may engage in other cellular processes such as mechanoelectric feedback and response to oxidative stress.
Pathways
NNav1.5 is an essential player in cardiac conduction and excitation-contraction coupling pathways. Within these pathways it interacts closely with proteins like calcium channels and potassium channels which regulate action potentials' phases and heart muscle contraction. These pathways show the intricate balance of ion exchanges responsible for cardiac rhythm and function highlighting nNav1.5's influence in the orderly contraction of the heart.
Mutations or dysfunctions in nNav1.5 can lead to cardiac arrhythmias and dilated cardiomyopathy. In conditions like Long QT Syndrome nNav1.5 mutations can cause delayed repolarization of the cardiac cells leading to irregular heartbeats. Another related disorder is Brugada syndrome where nNav1.5 malfunction leads to erratic electrical activity and a risk of sudden cardiac death. The interaction of nNav1.5 with other proteins like connexins affects these diseases as connexins influence gap junction communication essential for synchronized cardiac activity.