Abcam, ab6276, Anti-beta Actin antibody [AC-15] - Loading Control

Size: 50µL
Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody detecting beta Actin in Western Blot, ICC/IF . Suitable for African green monkey, Chinese hamster, Cow, Dog, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 2370 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AC-15,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P60709,
Epitope:N-terminal of the beta isoform of actin.

Product details:
Anti-beta Actin antibody [AC-15] (ab6276) is a mouse monoclonal antibody and is validated for use in ICC/IF and WB.
Anti-beta Actin antibody [AC-15] (ab6276) was first used in a scientific publication in 1994 and has been cited over 2378 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-beta Actin antibody [AC-15] (ab6276) has high sensitivity and specificity.
The specificity of Anti-beta Actin antibody [AC-15] (ab6276) has been confirmed by WB testing in beta Actin knockout HAP1 cells.
Anti-beta Actin antibody [AC-15] (ab6276) has 94 independent reviews from customers.
beta Actin antibodies are often used as loading controls in Western Blot. Anti-beta Actin antibody [AC-15] has been verified in Western Blot samples and detects a band at 42kDa Molecular weight.
Anti-beta Actin antibody [AC-15] (ab6276) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 50 µL selling sizes.
Antibody clone AC-15 is also available pre-conjugated to a variety of labels for your convenience - HRP, FITC (
ab6277
Beta-Actin (β-actin) is a highly conserved protein that plays a crucial role in cell motility, structure and integrity. It is one of the six actin isoforms found in humans and is a major component of the cytoskeleton in non-muscle cells. β-actin is involved in various cellular processes, including cell division, signaling and maintaining cell shape. Due to its ubiquitous expression and stability, β-actin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding β-actin's functions and interactions is essential for studying cellular dynamics and various physiological processes.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from hybridoma cell culture., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.
Biological function summary
Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.
Pathways
Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.
Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.