Abcam, ab63365, Anti-PKC theta/PRKCQ (phospho T538) antibody

Size: 100µg
Rabbit Polyclonal PKC theta/PRKCQ phospho T538 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCQ phospho T538 aa 500-600.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRKCQ phospho T538 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.Q04759,
Specificity:ab63365 detects PKC theta/PRKCQ only when phosphorylated at threonine 538.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PKC theta also known as PRKCQ is a protein kinase involved in signal transduction. The mass of PKC theta is approximately 85 kDa. It is a member of the protein kinase C (PKC) family which has distinctive roles in cellular processes. PKC theta specifically localizes in T-cells and is an important player in immune system functions. It is expressed predominantly in lymphoid tissues such as the spleen and thymus where T-cell activation and differentiation are significant.
Biological function summary
PKC theta participates in T-cell receptor signaling involved in the formation of immune synapses. It is an essential component of the multiprotein complex that includes molecules like LAT SLP-76 and Lck. PKC theta's activation allows the transmission of activation signals leading to the production of cytokines like IL-2. This function is integral to ensuring an effective immune response and the maintenance of immune homeostasis.
Pathways
PKC theta is central in the NF-kB pathway which transmits signals from T-cell receptors to the nucleus promoting gene transcription. It also plays a role in the AP-1 pathway collaborating with proteins such as CARMA1 and BCL10. These pathways underpin the immune response coordinating T-cell activation proliferation and survival linking PKC theta function to several immunological processes.
PKC theta has connections with autoimmune conditions such as rheumatoid arthritis and type 1 diabetes. In these disorders its role in T-cell signaling becomes dysregulated. This can lead to the overactive immune response against the body's tissues. Additionally PKC theta may interact with proteins involved in inflammatory signaling like NF-kB exacerbating inflammation and tissue damage. Understanding these relationships is important for developing therapies targeting immune dysregulation.