Abcam, ab64230, Mounting Medium for IHC - Aqueous

Size: 125mL
Aqueous Mounting medium (ab64230) is suitable for chromogenic tissue and cell staining in IHC applications for brightfield microscopy. Compatible with most HRP / peroxidase and alkaline phosphatase (AP) enzymatic substrates. - Works with both aqueous and non-aqueous chromogens (e.g., Fast-Red, AEC, DAB) - No coverslip necessary- forms a dry, even surface for direct visualization
Key facts
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.1% Sodium azide

Product details:
Mounting Medium ab64230 is an aqueous mounting medium designed for use with chromogenic tissue and cell staining. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.
Mounting media products
For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade
Fluorescence Mounting Medium ab104135
Mounting Medium with DAPI ab104139
Mounting Medium with PI ab104129
. For thick sections or tissues containing lots of fat, we recommend
Glycerol Mounting Medium with DAPI ab188804
For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend this product, Mounting Medium ab64230, or organic
Limonene Mounting Medium ab104141
IHC protocol suitable for use with Mounting Medium ab64230:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate
antigen retrieval buffer or enzyme
(primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough
hydrogen peroxide blocking solution
to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use
avidin biotin blocking
4. Apply
protein block
normal serum
from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in
antibody diluent
and incubate.
6. Wash 4 times in buffer. Incubate slide with
biotinylated secondary antibody
HRP polymer secondary antibody
and skip step 7). Wash 4 times in buffer.
7. Apply
streptavidin-HRP
and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in
DAB substrate
AEC Substrate
and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.
9. Add enough drops of
hematoxylin
to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add Mounting Medium ab64230 to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient