Abcam, ab64965, Anti-TRAP220/MED1 antibody

Size: 100µg
Anti-TRAP220/MED1 antibody (ab64965) is a rabbit polyclonal antibody detecting TRAP220/MED1 in Western Blot, IP, IHC-P, ELISA . Suitable for Human, Mouse . - Over 20 publications - Trusted since 2008
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:ELISA, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MED1. The exact immunogen used to generate this antibody is proprietary information.Q15648

Product details:
What is this antibody validated in?
Anti-TRAP220/MED1 antibody (ab64965) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA in Human, Mouse samples.
What is the molecular weight of TRAP220/MED1?
Anti-TRAP220/MED1 (ab64965) specifically detects a band for TRAP220/MED1 (UniProt: Q15648) at a molecular weight of 180kDa.
Trusted by the scientific community
Anti-TRAP220/MED1 (ab64965) was first used in a scientific publication in 2008 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-TRAP220/MED1 (ab64965) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRAP220 also known as MED1 is a protein with a molecular mass of approximately 220 kDa. It plays an important role as a component of the mediator complex which is important for transcription regulation. The expression of TRAP220/MED1 is found in various tissues with higher levels observed in liver lung and heart tissues. This protein acts mechanistically as a bridge between gene-specific transcription factors and RNA polymerase II facilitating the assembly of the pre-initiation complex.
Biological function summary
TRAP220/MED1 functions within the larger mediator complex a multi-protein coactivator essential for gene transcription by RNA polymerase II. It plays an important role in nuclear receptor signaling facilitating the transcriptional activation by various nuclear hormone receptors. MED1 interacts directly with these receptors through its various domains allowing the transcription of target genes responsive to hormonal signals.
Pathways
TRAP220/MED1 contributes significantly to transcriptional activation pathways involving nuclear receptors such as the PPAR (Peroxisome Proliferator-Activated Receptor) and thyroid hormone receptor pathways. It closely interacts with other proteins in these pathways including MED12 and MED14 which form integral parts of the mediator complex's function. Its involvement in these pathways highlights its critical role in lipid metabolism and energy homeostasis.
TRAP220/MED1 is associated with metabolic and proliferative diseases. Its altered expression or mutations can lead to conditions such as obesity and cancer. For instance aberrant MED1 activity or expression levels have connections to breast cancer development through its role in estrogen receptor-mediated gene expression. This correlation emphasizes the protein's potential impact in various pathophysiological contexts driving ongoing research into MED1 as a therapeutic target.