Abcam, ab6515, Anti-HSV2 ICP8 Major DNA binding protein antibody [4E6]

Size: 100µg
Mouse Monoclonal DNBI antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human herpesvirus 2 samples. Cited in 1 publication. Immunogen corresponding to Virus preparation containing HSV2 protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4E6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human herpesvirus 2,
Applications:WB, ICC/IF, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HSV2 ICP8 Major DNA binding protein also known as infected cell protein 8 or ICP8 plays an important role in the replication machinery of the herpes simplex virus type 2 (HSV-2). ICP8 is a single-stranded DNA-binding protein with an approximate mass of 128 kDa. It expresses in large quantities in cells infected by HSV-2 facilitating the assembly of other viral proteins needed for effective viral DNA replication. The protein's structure allows it to bind DNA strongly and support the viral DNA replication process.
Biological function summary
ICP8 interacts with other viral and cellular proteins to organize the replication compartment inside the host cell nucleus. It forms part of the replication complex working alongside HSV-2 DNA polymerase and other accessory proteins to effectively duplicate the viral genome. ICP8 facilitates the unwinding of DNA and stabilizes the single-stranded conformation ensuring that the replication is efficient. Its ability to bind single-stranded DNA and engage with various partners highlights its importance in managing viral replication dynamics.
Pathways
ICP8 is central to the HSV-2 replication and DNA synthesis pathways. The protein acts in concert with viral proteins such as UL42 and HSV DNA polymerase to enhance replication efficiency. The DNA repair pathway also sees involvement by ICP8 allowing interplay with cellular mechanisms to support or hinder the cellular response to viral assault. ICP8 ensures that HSV-2 effectively commandeers the host's cellular machinery to prioritize viral genome amplification.
HSV2 ICP8 Major DNA binding protein contributes strongly to the pathogenicity of HSV-2 which causes genital herpes. The protein's function in virus replication makes it an important part of the infection process and it finds connections to proteins like UL29 and UL5 which also contribute to the virus's infectious cycle. Understanding its interaction with these viral proteins helps unravel the pathogenesis of HSV-2 leading to better-targeted therapies for managing infections.