Abcam, ab65346, Ascorbic Acid Assay Kit

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Ascorbic Acid Assay Kit ab65346 uses a non-enzyme catalyst to oxidise ascorbic acid, followed by interaction with a probe. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Tissue Extracts, Cell culture supernatant, Other biological fluids,
Assay type:Quantitative,
Range:0.01 - 10 nmol/well,
Assay time:30m,
Assay Platform:Microplate reader

Product details:
Ascorbic Acid Assay Kit ab65346 provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples.
In the ascorbic acid assay protocol, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid levels can be easily determined by either colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) methods.
The assay can detect 0.01-10 nmol of ascorbic acid per assay.
Related Ascorbic Acid assays
- For plasma and serum samples, we recommend Ascorbic Acid Assay Kit (Colorimetric)
ab65656
, which uses an iron reduction method combined with ascorbate oxidase
- Ascorbic Acid Assay Kit (Fluorometric)
ab219928
uses an alternative method based on ascorbate oxidase
Other Notes
This product was previously called K661 Biovision Ascorbic Acid Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ascorbic acid also known as vitamin C plays an important mechanical role as an antioxidant and a cofactor in various enzymatic reactions. It is water-soluble and has a molecular mass of approximately 176.12 g/mol. Ascorbic acid is widely expressed in fruits and vegetables and cells in both animals and plants. Inside cells it primarily exists as ascorbate which can undergo oxidation by the enzyme ascorbate oxidase relevant in some assay tools used in biology research.
Biological function summary
As a potent reducing agent ascorbic acid is critical for the hydroxylation of collagen which is essential for connective tissue strength in humans and animals. It also participates in the synthesis of carnitine involved in fat metabolism and acts in the biosynthesis of neurotransmitters. Ascorbic acid forms a part of antioxidant defense complexes working alongside enzymes like glutathione peroxidase to protect cells against oxidative stress.
Pathways
Ascorbic acid is integral in the citrate cycle (TCA cycle) and the pentose phosphate pathway. It interacts with proteins such as prolyl hydroxylase involved in collagen synthesis within the TCA cycle. The pentose phosphate pathway helps produce NADPH which works with ascorbic acid in maintaining cellular antioxidant status supporting its role in the reduction of oxidized forms of essential biomolecules.
Ascorbic acid deficiency leads to scurvy a condition characterized by weakened connective tissue leading to symptoms like bleeding gums and joint pain. It also plays a role in diabetes with oxidative stress being an underlying mechanism linked to the disease. Ascorbic acid's connection to oxidative damage involves proteins like advanced glycation end-products found in diabetic complications where its antioxidant properties help mitigate related tissue damage.