Abcam, ab65771, Anti-GRC5 / PHF2 antibody

Size: 100µL
Rabbit Polyclonal GRC5 / PHF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PHF2 aa 800 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PHF2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O75151

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRC5 also known as PHF2 is a protein that functions as a JmjC domain-containing histone demethylase. It specifically demethylates lysine residues on histone H3K9me1 and H3K9me2. This protein has a mass of approximately 125 kDa and expresses widely in human tissues including the liver and brain. The ability of PHF2 to alter chromatin structure makes it a significant regulator of gene expression.
Biological function summary
PHF2 interacts with chromatin remodeling complexes to facilitate transcriptional activation. It is part of the SIRT1-HDAC complex which affects gene expression by modifying the acetylation status of histones and non-histone proteins. By demethylating histones PHF2 plays a role in cellular stress response and metabolic regulation impacting processes like gluconeogenesis and inflammation.
Pathways
PHF2 integrates into transcriptional regulatory networks by modulating epigenetic marks. It participates in the circadian rhythm and metabolic pathways along with related proteins such as CLOCK and ARNTL (BMAL1). These interactions ensure the proper timing of gene expression in response to environmental changes essential for maintaining metabolic homeostasis.
PHF2 relevance is noticeable in metabolic disorders and certain cancers. Aberrant expression or mutations in PHF2 associates with obesity and breast cancer. The protein interacts with pathways involving p53 and SIRT1 which play roles in DNA repair and cell survival pathways implicating PHF2 in the cellular response to oncogenic stress and metabolic imbalance.