Abcam, ab6586, Anti-Collagen IV antibody

Size: 100µg
Anti-Collagen IV antibody (ab6586) is a rabbit polyclonal antibody detecting Collagen IV in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for African green monkey, Cow, Dog, Hamster, Human, Mouse, Pig, Rat, Zebrafish . - Over 920 publications - Trusted since 2001
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Hamster, Syrian hamster, Cow, Mouse, Dog, Zebrafish, Rat, Chinese hamster, African green monkey, Pig,
Applications:ICC/IF, IF, IHC-FoFr, IHC-P, IP, ELISA, WB, IHC-FrFl, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Product details:
Anti-Collagen IV antibody (ab6586) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IF, IHC-FoFr, IHC-Fr, IHC-FrFl,
Anti-Collagen IV antibody (ab6586) was first used in a scientific publication in 2004 and has been cited over 920 times in peer reviewed journals. It's performance in IHC and Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen IV antibody (ab6586) has high sensitivity and specificity.
Anti-Collagen IV antibody (ab6586) has 120 independent reviews from customers.
Anti-Collagen IV antibody (ab6586) specifically detects Collagen IV (UniProt ID: P02462; Molecular weight: ~160kDa) and is sold in 100 µg selling sizes.
Collagen IV is a key component of the extracellular matrix in tumors, contributing to tissue stiffness and promoting cancer cell invasion. It plays a significant role in tumor progression and metastasis, influencing the behaviour of cancer cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen IV also known as COL4A1 and collagen type 4 plays a significant mechanical role in the structure of the basement membrane. It provides structural support and mechanical stability. Collagen IV has a molecular weight ranging approximately from 160 to 240 kDa. It is expressed in various regions including the kidney lung and vascular tissues where it contributes to the integrity and function of these organs.
Biological function summary
The primary function of collagen type IV involves forming a scaffold essential for basement membrane assembly and function. It exists as a part of a complex formation typically forming networks with other collagens and proteins. These networks are important for cellular adhesion differentiation migration and filtration processes. Collagen IV interacts with different cell surface receptors helping mediate important cellular signals.
Pathways
Collagen type 4 is integral to the extracellular matrix and integrin-mediated signaling pathways. It partners with proteins such as laminins and nidogens in these pathways contributing to cell adhesion and migration. This interaction helps regulate various cellular processes including wound healing and tissue repair. By being a part of these pathways collagen IV impacts cellular behavior and tissue homeostasis.
Dysfunction or mutations in collagen IV relate to conditions such as Alport syndrome and Goodpasture's syndrome. Alport syndrome involves mutations in collagen IV leading to kidney dysfunction and hearing loss. In Goodpasture's syndrome collagen IV becomes a target for autoimmune attack resulting in damage to the lungs and kidneys. These diseases illustrate the connection between collagen IV and other proteins like laminin which together contribute to maintaining tissue integrity and preventing disease progression.