Abcam, ab66645, Anti-HIV1 integrase antibody [IN-2]

Size: 100µL
Mouse Monoclonal POL antibody. Suitable for WB, IP, ICC/IF and reacts with Human immunodeficiency virus samples. Cited in 12 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:IN-2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human immunodeficiency virus,
Applications:IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:ab66645 is specific for bacterial HIV1 integrase (IN) protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV-1 integrase also known simply as integrase is an enzyme that acts mechanically by catalyzing the insertion of viral DNA into the host DNA. It is essential for the replication cycle of HIV-1 the virus responsible for AIDS. The molecular mass of the integrase protein is approximately 32 kDa. Expression of integrase occurs within the viral particle and is integral to the viral genome. Integrases allow HIV-1 to integrate its genetic material into the DNA of host cells a vital step for viral replication.
Biological function summary
HIV-1 integrase operates as a part of the pre-integration complex (PIC) within infected host cells. This enzyme facilitates two consecutive reactions: 3'-end processing and strand transfer. The 3'-end processing involves trimming the ends of viral DNA and strand transfer involves the integration of viral DNA into host genomic DNA. The activity of integrase needs to be coordinated with other viral proteins such as reverse transcriptase to ensure successful integration and replication cycles.
Pathways
Integrase is an important component in the retroviral integration pathway which is important for the proliferation of HIV-1 within the host. This pathway includes steps that involve other viral proteins such as reverse transcriptase and protease. Reverse transcriptase synthesizes viral DNA from RNA which integrase then incorporates into the host DNA. The integrase pathway interplays with host cellular mechanisms and manipulates them to benefit viral replication.
HIV-1 integrase is associated with the progression of HIV/AIDS. Inhibiting this protein effectively blocks the integration step preventing new infections of host cells and is an established therapeutic approach in antiretroviral treatments. Integrase inhibitors such as raltegravir specifically target this enzyme illustrating its pivotal role in disease management for HIV/AIDS patients. By targeting HIV integrase treatment regimens aim to disrupt the life cycle of HIV-1 and suppress viral loads in patients.