Abcam, ab68260, Anti-Cytokeratin 3/CK-3 antibody [AE5]

Size: 100µg / 1mg
Mouse Recombinant Monoclonal K2C3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 40 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AE5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.

Product details:
This product has switched from a hybridoma to recombinant production method on 5
January 2022

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 3 also known as CK-3 or CK3 is a type II cytokeratin protein with a molecular weight of approximately 64 kDa. This protein often coexists with Cytokeratin 12 and together they form the epithelial keratins. CK-3 is expressed predominantly in corneal epithelial cells. The presence of CK-3 in these cells helps maintain structural integrity and resilience against mechanical stress through its network of intermediate filaments. Various forms like CK-3 IgG and IGG CK3 have been studied to understand the unique and important role of cytokeratins in epithelial tissues.
Biological function summary
CK-3 plays an important role in the structural framework inside corneal epithelial cells. It participates in forming a complex of intermediate filaments providing necessary strength and stability to the cells. Such a framework is important for protecting the epithelium under physical stressors while also supporting the dynamic processes of cell proliferation and differentiation. These cytokeratin complexes contribute to the cellular process by supporting the resilience of epithelial cells.
Pathways
The involvement of CK-3 in cytoskeletal organization highlights its connection to certain critical signaling pathways such as the epithelial-to-mesenchymal transition (EMT) and cell differentiation pathways. The interaction of CK-3 with proteins like Cytokeratin 12 regulates these pathways and contributes to maintaining corneal epithelial homeostasis and integrity. Such interactions enable CK-3 to help maintain the proper functioning and regeneration of epithelial barriers.
CK-3's alteration or dysfunction may contribute to corneal-related diseases such as keratitis and corneal dystrophies. Abnormalities or mutations in CK-3 affect corneal integrity leading to compromised vision and disease symptoms. In these disorders its interaction with proteins like AE5 which participates in corneal structural pathways can be disrupted leading to pathophysiological conditions affecting the corneal epithelium. Understanding CK-3's role in these diseases may help guide therapeutic strategies.