Abcam, ab69357, Anti-RNF220 antibody

Size: 50µg
Mouse Polyclonal RNF220 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNF220.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RNF220.Q5VTB9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF220 also known as Ring Finger Protein 220 is an E3 ubiquitin-protein ligase. It has a molecular weight of approximately 45 kDa. As a member of the RING finger protein family RNF220 participates in the process of attaching ubiquitin molecules to target proteins which usually marks them for degradation by the proteasome. This protein is broadly expressed in human tissues with significant expression in the testis ovaries and brain.
Biological function summary
RNF220 plays a role in the regulation of transcription factors and is part of certain protein complexes. It interacts with the ubiquitination machinery to control the stability and activity of various transcriptional regulators influencing gene expression patterns essential for cellular differentiation and development. This protein contributes to the regulation of right/left asymmetry in embryonic development through its interaction with key developmental transcription factors.
Pathways
RNF220 plays a role in the Wnt signaling pathway and the Hedgehog signaling pathway. Through these pathways it influences cellular proliferation and differentiation. It interacts with proteins such as β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway. These interactions regulate the transcriptional output of these signaling cascades influencing processes like embryogenesis and tissue homeostasis.
RNF220 links to developmental disorders and certain cancers. Mutations or dysregulation of RNF220 can contribute to conditions such as congenital asymmetry disorders through its interaction with transcription factors like Pitx2. Additionally its role in the Wnt and Hedgehog pathways implicates it in cancers including medulloblastoma where it interacts with proteins involved in regulating cell cycle and apoptosis.