Abcam, ab69506, Anti-PDGFR beta antibody [42G12]

Size: 100µg
Anti-PDGFR beta antibody [42G12] (ab69506) is a mouse monoclonal antibody detecting PDGFR beta in Western Blot, Flow Cytometry, IHC-P, ELISA . Suitable for Human, Mouse . - KO validated for confirmed specificity - Over 40 publications - Trusted since 2008
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:42G12,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, I-ELISA, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PDGFRB.P09619

Product details:
This product was changed from ascites to tissue culture supernatant on 22
May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Product Specifications
Anti-PDGFR beta antibody [42G12] (ab69506) is a mouse monoclonal antibody and is validated for use in Flow Cyt, I-ELISA, IHC-P, WB in human, mouse samples.
Anti-PDGFR beta antibody [42G12] (ab69506) specifically detects PDGFR beta (UniProt ID: P09619; Molecular weight: 121kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-PDGFR beta antibody [42G12] (ab69506) has high sensitivity and specificity.
The specificity of Anti-PDGFR beta antibody [42G12] (ab69506) has been confirmed by testing in knockout samples.
Anti-PDGFR beta antibody [42G12] (ab69506) has been cited over 44 times in peer reviewed journals and is trusted by the scientific community.
Anti-PDGFR beta antibody [42G12] (ab69506) has 4 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone 42G12 -
ab202760

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
Biological function summary
The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.
Pathways
PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.
PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.