Abcam, ab70269, Anti-DHX36 antibody

Size: 50µL
Rabbit Polyclonal DHX36 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human DHX36 aa 950 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DHX36 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9H2U1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DHX36 also known as RHAU or G4R1 is a member of the DEAH-box family of RNA helicases. It has an estimated molecular mass of approximately 115 kDa. The protein exhibits robust expression in various tissues including the heart skeletal muscle and brain. Mechanically DHX36 plays a role in resolving RNA G-quadruplexes and DNA G-quadruplexes which are secondary structures that can form in nucleic acids. Its ability to unwind these structures is critical for the regulation of gene expression and maintenance of genomic stability.
Biological function summary
DHX36 acts as an RNA helicase and is essential in the regulation of translation and transcription. It plays a significant role in binding G-quadruplex structures within the 5' untranslated region of mRNAs. DHX36 also forms part of larger protein complexes that regulate ribosome assembly and function. This protein helps ensure proper ribosomal RNA processing facilitating efficient protein synthesis. Through its involvement in these complexes DHX36 aids in the regulation of cellular stress responses and apoptosis.
Pathways
DHX36 significantly contributes to several cellular processes notably the regulation of transcription and translation. It actively participates in the Wnt signaling pathway influencing the stability of target mRNAs. DHX36 also interacts with proteins like FTO and NPM1 that are known to be involved in mRNA processing. These interactions and pathway linkages highlight its role in controlling gene expression and cellular growth.
DHX36 has been linked to cardiac dysfunctions and neurological disorders. Its improper functioning has been associated with arrhythmias due to its role in cardiac gene expression regulation. In neurological contexts DHX36 disruptions may contribute to various cognitive disorders acting through its impact on synaptic protein synthesis. In these pathological conditions proteins such as TDP-43 and FUS are affected emphasizing the importance of DHX36 in maintaining cellular homeostasis and preventing disease progression.