Abcam, ab70311, Anti-EDD antibody

Size: 100µL
Rabbit Polyclonal EDD antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human UBR5.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human UBR5. The exact immunogen used to generate this antibody is proprietary information.O95071

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70311 was affinity purified using an epitope specific to EDD immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDD also known as EDD1 or EDDC is a significant target within cellular processes. This protein possesses a molecular mass of approximately 300 kDa and functions as a ubiquitin-protein ligase. EDD is highly expressed in many tissues with noticeable levels in reproductive tissues brain and pancreas. It plays a mechanical role in tagging proteins for degradation via the ubiquitin-proteasome pathway contributing to protein turnover and regulation.
Biological function summary
EDD or EDDC acts as a central regulator of cell cycle progression and DNA damage response. It is part of the complex known as EDD-BA where it interacts with other proteins to control cellular processes. One main function of EDD lies in mediating the degradation of proteins preventing the accumulation of destructive or malfunctioning proteins that could harm cell integrity. Through its ligase activity EDD modifies target proteins with ubiquitin guiding them toward their respective cellular fates.
Pathways
EDD interacts with several essential cellular pathways including the cell cycle and the TGF-beta signaling pathway. In the cell cycle pathway EDD collaborates with proteins like p53 to ensure proper cell cycle arrest and DNA repair when damage is detected. The involvement of EDD in the TGF-beta pathway highlights its role in cellular growth and apoptosis. Through these interactions EDD maintains cellular order and helps respond to cellular stress and damage.
EDD has associations with cancer and developmental disorders. Abnormalities in EDD expression or function can lead to uncontrolled cell proliferation linking it to various cancers. Its interaction with p53 reinforces this connection as p53 mutations are common in cancers. Furthermore mutations in EDD have been implicated in developmental disorders where disrupted protein regulation impacts cellular growth and differentiation processes. Understanding EDD's role in these contexts provides insights into therapeutic targets and disease mechanisms.