Abcam, ab70346, Anti-Nucleostemin antibody

Size: 100µL
Rabbit Polyclonal Nucleostemin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Mouse, Human, Rat, Chicken samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human GNL3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Chicken, Rat, Human,
Applications:IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GNL3. The exact immunogen used to generate this antibody is proprietary information.Q9BVP2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab70346 was affinity purified using an epitope specific to Nucleostemin immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nucleostemin is a protein involved in cellular regulation and has an approximate mass of 60 kDa. It is also known by the alternative name GNL3. Mechanical function of nucleostemin involves involvement in the regulation of cell cycle and maintenance of stem cell properties. This protein is expressed mainly in the nucleolus and is found in abundance within stem cells and certain cancer cells where its levels change depending on cell cycle stages.
Biological function summary
Nucleostemin plays an important role in controlling cell proliferation and maintaining stem cell characteristics. It is not part of a large complex but interacts with other proteins to perform its functions. The protein facilitates the regulation of cell division and its presence in stem cells assures the maintenance of an undifferentiated state which is important for tissue regeneration and repair.
Pathways
Nucleostemin interacts with various cell cycle and DNA repair mechanisms. Among important biological pathways nucleostemin is involved in the p53 signaling pathway assisting in cellular stress responses and enabling cell survival during DNA damage. There is also a relationship with the MDM2 protein as nucleostemin indirectly influences p53 stability by modulating MDM2 activity therefore playing a role in tumor suppression and oncogenic processes.
Researchers associate nucleostemin with cancer and neurological disorders. In cancers its overexpression is linked to the unchecked proliferation of tumor cells often observed in glioblastoma and certain sarcomas where nucleostemin interacts with proteins like p53 and MDM2 to influence tumor dynamics. In neurological contexts its role in stem cell regulation draws attention when considering diseases involving neurodegeneration where maintaining neural stem cells remains important.