Abcam, ab70549, Anti-Rbm15/OTT antibody

Size: 100µL
Rabbit Polyclonal Rbm15/OTT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 11 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rbm15 also known as OTT is a multifunctional RNA-binding protein with a mass of approximately 82 kDa. This protein participates in various cellular processes by binding to RNA molecules influencing their processing and stability. It is expressed in numerous tissues including hematopoietic cells in the bone marrow and fetal liver. The characteristic expression indicates its importance in developmental and cellular contexts.
Biological function summary
Rbm15 plays a role in the regulation of mRNA splicing and transcriptional activation. It functions as part of the methyltransferase complex which involves the methylation of mRNA as a post-transcriptional modification contributing to RNA stability and translation efficiency. This action suggests its involvement in gene expression regulation and cellular differentiation.
Pathways
Rbm15 interacts closely with the Notch signaling pathway which is significant for cell fate determination and differentiation. Rbm15 partners with other proteins such as Numb to mediate repression or activation of Notch-related transcriptional activity. Also Rbm15 affects signaling pathways through interactions with Wnt proteins which play an important role in embryonic development and cell proliferation.
Rbm15 has associations with acute megakaryoblastic leukemia (AMKL) and myelodysplastic syndromes (MDS). In AMKL Rbm15 forms a fusion protein with MKL1 resulting from chromosomal translocations which contributes to leukemogenesis. Understanding its interaction with MKL1 and other fusion partners provides insight into the pathogenesis of these hematological disorders. The study of Rbm15's role in these contexts holds potential for therapeutic advances.