Abcam, ab70731, Anti-E2F2 antibody [2302C4a]

Size: 100µg
Mouse Monoclonal E2F2 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E2F2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2302C4a,
Isotype:IgG2a,
Carrier free:No,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human E2F2. The exact immunogen used to generate this antibody is proprietary information.Q14209

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab70731 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane., Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
E2F2 also known as E2F transcription factor 2 is a member of the E2F family of transcription factors. It is a protein with a mass of approximately 46 kDa. It plays an important role by regulating the expression of genes involved in cell cycle progression particularly the transition from G1 to S phase. Researchers have identified that E2F2 is expressed in various tissues with high expression noted in actively dividing cells such as those in fetal tissues adult testis and tumor cells.
Biological function summary
E2F2 functions as a transcriptional activator and repressor controlling genes necessary for DNA synthesis and cell cycle progression. It often associates with retinoblastoma protein (RB1) to form complexes that finely regulate gene transcription. E2F2 not only controls cell proliferation but also influences cell differentiation processes by coordinating the balance between distinct cellular states.
Pathways
E2F2 interacts with key players in the cell cycle such as cyclins and cyclin-dependent kinases (CDKs). It is an integral part of the Rb-E2F signaling pathway which governs cell cycle checkpoints and progression. This pathway's regulation heavily influences the activities of other E2F family members such as E2F1 and E2F3 which can either promote or inhibit cell cycle transitions in response to various signals.
E2F2's dysregulation associates with cancer and retinoblastoma. Uncontrolled E2F2 activity often contributes to tumorigenesis by bypassing cell cycle controls resulting in unchecked cell division. In cancer altered interactions between E2F2 and proteins like RB1 can lead to the loss of tumor suppressor functions such as those observed in certain aggressive tumors. Understanding these interactions can provide insights into therapeutic targets for cancer treatment.