Abcam, ab70821, Anti-DDX28 antibody

Size: 100µL
Rabbit Polyclonal DDX28 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DDX28.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human DDX28. The exact immunogen used to generate this antibody is proprietary information.Q9NUL7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DDX28 protein also known as DEAD box helicase 28 is a member of the DEAD box protein family. Its molecular mass is approximately 57 kDa. This family of proteins is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX28 functions as an ATP-dependent RNA helicase which means it unwinds RNA structures and plays roles in rearranging ribonucleoprotein complexes. This protein is expressed in several tissues with significant expression in the liver and heart with the potential for further tissue-specific investigations.
Biological function summary
DDX28 participates in RNA processing and mitochondrial ribosome biogenesis activities. It is known to be a component of the mitoribosome a specialized ribosome for mitochondria. Being part of the mitoribosome DDX28 assists in the synthesis and assembly of proteins that are encoded by the mitochondrial genome. As helicases generally support the proper assembly and function of these complexes DDX28's role becomes important for maintaining mitochondrial translation integrity and efficiency.
Pathways
DDX28 interacts with several components within mitochondrial translation and RNA processing pathways. It associates notably with other mitochondrial ribosomal proteins forming a connection to the larger mitochondrial protein synthesis and assembly pathway. This connection is essential for the translation of mitochondrially-encoded proteins. DDX28 also has potential links with other helicase proteins such as DDX3X which leads to the interplay within various RNA helicase-involved pathways contributing to RNA metabolism and homeostasis in cells.
Mutations or dysregulation of DDX28 have been connected to mitochondrial dysfunction disorders including mitochondrial biogenesis deficiencies which impacts cellular energy homeostasis and can lead to serious conditions. Its involvement with other helicases such as DHX30 is also significant in certain neurodegenerative disorders. These associations highlight how alterations in DDX28's function can significantly affect cellular physiology and cascade into disease states when mitochondrial protein synthesis is impaired.