Abcam, ab70842, Anti-MED12 antibody

Size: 100µL
Rabbit Polyclonal MED12 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MED12 aa 2150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MED12 aa 2150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q93074

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MED12 also known as mediator complex subunit 12 is a component of the transcriptional Mediator complex. It weighs approximately 250 kDa and is widely expressed in many tissues showing high transcriptional regulatory activity. MED12 collaborates with other subunits within the Mediator complex to transmit signals from regulatory DNA elements to RNA polymerase II facilitating gene expression regulation.
Biological function summary
MED12 functions as a core component of the larger Mediator complex which plays an important role in controlling RNA polymerase II-dependent genes. Through interactions with activators and repressors MED12 participates in chromatin modification and looping affecting gene transcription rates. It is enriched in tissues where fine control of gene expression is essential impacting growth differentiation and development processes.
Pathways
MED12 integrates into critical signaling pathways including the Wnt/β-catenin and Hedgehog pathways. In these pathways MED12 interacts with other proteins like β-catenin serving as a connecting node that influences the transcription of target genes essential for cell proliferation and differentiation. Such interactions help coordinate cellular responses to external signals and ensure proper pathway regulation.
MED12 mutations have been linked to disorders like Lujan-Fryns syndrome and uterine leiomyomas. In these contexts MED12 interacts with G protein-coupled receptors and other transcription factors such as β-catenin resulting in dysregulation that leads to the development of these conditions. Understanding the connections between MED12 and associated proteins provides insights into the underlying mechanisms of these pathologies.