Abcam, ab71333, Anti-Talin 1 antibody

Size: 100µg
Knockout Tested Rabbit Polyclonal Talin 1 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse, Human samples. Cited in 44 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab71333 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab108480.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.
Biological function summary
Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.
Pathways
Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.
Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.