Abcam, ab7255, Anti-68kDa Neurofilament/NF-L antibody [DA2]

Size: 250µL
Mouse Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 17 publications. Immunogen corresponding to Native Full Length Protein corresponding to Pig NEFL.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:DA2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Pig NEFL.P02547,
Epitope:Epitope mapped to a short peptide in the C-terminal “tail” region of the molecule within the human sequence SYYTSHVQEEQIEVE, amino acids 441-455.,
Specificity:Specifically recognizes the light neurofilament subunit or 68kDa Neurofilament/NF-L.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.033% Sodium azideConstituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
Biological function summary
The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.
Pathways
Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.
Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.