Abcam, ab72592, Anti-Sarcomeric Alpha Actinin antibody

Size: 100µg
Rabbit Polyclonal Sarcomeric Alpha Actinin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human ACTN2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ACTN2. The exact immunogen used to generate this antibody is proprietary information.P35609

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.
Biological function summary
Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.
Pathways
Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.
Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.