Abcam, ab7291, Anti-alpha Tubulin antibody [DM1A] - Loading Control

Size: 100µg / 250µg
Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) is a mouse monoclonal antibody detecting alpha Tubulin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 1220 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:DM1A,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.
Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) was first used in a scientific publication in 2005 and has been cited over 1224 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has high sensitivity and specificity.
Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) has 91 independent reviews from customers.
alpha Tubulin antibodies are often used as loading controls in Western Blot. Anti-alpha Tubulin antibody [DM1A] - Loading Control has been verified in Western Blot samples and detects a band at 51kDa Molecular weight.
Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) specifically detects alpha Tubulin (UniProt ID: P02552; Molecular weight: 51kDa) and is sold in 100 µg and 250 µg selling sizes.
Conjugation-ready, carrier free format available for antibody clone DM1A -
ab264493
Antibody clone DM1A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
68, Alexa Fluor
79, Alexa Fluor
488, Alexa Fluor
594, HRP, FITC (
ab18493
ab184577
ab195887
ab195889
ab4742
Alpha-Tubulin (α-Tubulin, TUBBA) is a fundamental protein that forms microtubules, essential components of the cytoskeleton in eukaryotic cells. Microtubules, composed of α- and β-tubulin heterodimers, play a crucial role in maintaining cell shape, enabling intracellular transport and facilitating cell division. Due to its ubiquitous presence and stability, α-Tubulin is commonly used as a loading control in western blotting (WB) and other protein quantification assays. Understanding α-Tubulin's functions and interactions is vital for studying cellular dynamics, mitosis and various physiological processes

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Affinity-purified using protein G, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.
Biological function summary
Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.
Pathways
Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.
Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.