Abcam, ab72962, Anti-RAG2 antibody

Size: 50µg
Mouse Polyclonal RAG2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAG2_HUMAN.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RAG2_HUMAN.P55895

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAG2 also known as Recombination Activating Gene 2 plays an important role in the mechanism of V(D)J recombination aiding in the assembly of T-cell receptors and immunoglobulins. It acts together with RAG1 as a heterodimer complex. RAG2 lacks enzymatic activity but assists in this complex by binding to RAG1 facilitating recombinase activity. The molecular weight of RAG2 is around 56 kDa and it is mainly expressed in developing lymphocytes within the thymus and bone marrow.
Biological function summary
RAG2 contributes to lymphocyte development by enabling the production of diverse antigen receptor repertoires. It acts as a non-catalytic partner with RAG1 forming a complex necessary for the cleavage of DNA at specific recombination signal sequences. This complexity is fundamental in generating the variability of antibodies and T-cell receptors. RAG2 alters the chromatin structure enhancing accessibility to the recombination substrates and ensuring efficient gene rearrangement.
Pathways
RAG2 is essential in the adaptive immune response being vital to V(D)J recombination in lymphocyte development. It operates extensively in the antigen receptor gene assembly pathway influencing B cell development and T cell maturation. Throughout these processes RAG2 works closely with proteins like RAG1 where both are co-dependent for functional recombinase activity essential for immune system adaptability.
Mutations or malfunctions in RAG2 can lead to immunodeficiency syndromes such as severe combined immunodeficiency (SCID) and Omenn syndrome. These disorders often stem from defective or inefficient V(D)J recombination causing improper immune cell function. RAG2's relationship with RAG1 is critical as defects in this complex impair lymphocyte development leading to reduced immune response capability aligned with these diseases.