Abcam, ab733, Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311]

Size: 250µL
Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733) is a mouse recombinant multiclonal antibody detecting Melanoma in Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF . Suitable for Human . - Recombinant format for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Multiclonal,
Clone number:HMB45 + M2-7C10 + M2-9E3 + T311,
Isotype:IgG,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, IHC-P, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas.MART-1 recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A.Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions.Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas.Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45.

Product details:
What is this antibody validated in?
Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733) is a mouse recombinant recombinant multiclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-Melanoma [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Antibodies in this cocktail- HMB45, MART-1 and Tyrosinase are affinity purified., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle