Abcam, ab735, Anti-p63 antibody [4A4]

Size: 250µL
Anti-p63 antibody [4A4] (ab735) is a mouse monoclonal antibody detecting p63 in IHC-P . Suitable for Human . - Over 150 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4A4,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Mouse Tp63 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.O88898

Product details:
Please note that clone [BC4A4] and clone [4A4] are identical.
This product was changed from ascites to tissue culture supernatant on 14/07/17. The following lots are from ascites and are still in stock as of 14/07/17 – GR323232, GR293168, and GR301030. Lot numbers higher than GR323232 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Product Specifications
Anti-p63 antibody [4A4] (ab735) is a mouse monoclonal antibody and is validated for use in IHC-P in human samples.
Anti-p63 antibody [4A4] (ab735) specifically detects p63 (UniProt ID: Q9H3D4; Molecular weight: 77kDa) and is sold in 250 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-p63 antibody [4A4] (ab735) has high sensitivity and specificity.
Anti-p63 antibody [4A4] (ab735) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.
Anti-p63 antibody [4A4] (ab735) has 12 independent reviews from customers.
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Conjugation-ready, carrier free format available for antibody clone 4A4 -
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Protein A/G affinity column, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p63 protein also known as TP63 or KET is a transcription factor that plays a role in the regulation of cell cycle and apoptosis. With a molecular mass of approximately 77 kDa it expresses predominantly in epithelial tissues such as those in the skin breast and prostate. The expression pattern of p63 makes it a useful marker in developmental biology and cancer diagnosis. Antibodies like '4A4' specifically recognize p63 in various assays including immunohistochemistry.
Biological function summary
P63 participates in the control of epithelial cell differentiation and proliferation. It functions as a member of the p53 family which includes p53 and p73. This family forms an important part of the complex regulatory network that maintains genomic stability. Research using methods like p63 staining in immunohistochemistry highlights its critical role in stratified epithelia. The gene encodes several isoforms with different biological activities impacting processes such as cellular senescence and development.
Pathways
P63 integrates into various signaling pathways that influence cellular growth and adherence. It notably impacts the Notch and Wnt pathways both of which are critical for developmental processes and cancer progression. Through these pathways p63 interacts with proteins like β-catenin and Notch receptors facilitating cross-talk that influences cell fate decisions. Its function relates closely to other transcription factors forming feedback loops that reinforce its regulatory impact.
P63 shows strong associations with ectodermal dysplasia and different types of cancers like squamous cell carcinoma. Mutations and altered expressions of p63 contribute to these pathologies by disrupting normal cell proliferation and differentiation. The protein interacts closely with p73 in cancer suggesting a compensatory role when p63 function is compromised. Understanding these connections can guide the development of targeted therapies and improve diagnostic accuracy.