Abcam, ab73984, Anti-PNGase antibody

Size: 200µL
Rabbit Polyclonal PNGase antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NGLY1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.Q96IV0

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PNGase also known as Peptide:N-Glycosidase F is an enzyme that mechanically removes N-linked glycans from glycoproteins a process known as deglycosylation. PNGase primarily acts by cleaving the bond between the asparagine residue of the peptide and the N-linked oligosaccharides. The enzyme is extensively utilized in laboratories where it has become important in the study of glycoprotein characterization and analysis. PNGase F with a molecular mass of approximately 36 kDa is expressed in the bacterium Flavobacterium meningosepticum and sometimes can be recombinantly expressed in hosts like E. coli for research purposes.
Biological function summary
PNGase F plays a significant role in the modification of proteins and affects their stability solubility and function. It operates as a part of the broader glycan-processing machinery but is not typically within the complex protein structures. By removing glycan groups PNGase F influences protein folding and degradation processes making it key in the study of protein lifespan and function after glycosylation. Understanding its activity assists in elucidating the complex roles of glycoproteins in cellular function and signaling.
Pathways
PNGase F is involved in glycoconjugate pathway modulation by facilitating the turnover of glycoproteins. It interfaces with the ERAD (Endoplasmic Reticulum-Associated Degradation) pathway by preparing misfolded glycoproteins for further degradation. Through these interactions PNGase F indirectly influences proteins like EDEM (ER degradation-enhancing alpha-mannosidase-like protein) which aid in the recognition and targeting of proteins for disposal within the cell ensuring the cellular protein quality control.
PNGase F activity relates predominantly to neurodegenerative diseases like Alzheimer's where abnormal glycoprotein accumulation occurs. Abnormal glycans or improperly folded glycoproteins contribute to disease pathology and PNGase F's functional relationship with ERAD affects proteins involved in misfolding such as amyloid precursor protein (APP). This connection suggests that PNGase F could play a potential role in both understanding and developing therapeutic strategies for diseases where glycoprotein metabolism is disrupted.