Abcam, ab74091, Anti-ACK1 (phospho Y284) antibody

Size: 100µg
Rabbit Polyclonal ACK1 phospho Y284 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TNK2 phospho Y284.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TNK2 phospho Y284. The exact immunogen used to generate this antibody is proprietary information.Q07912

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab74091 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACK1 also known as TNK2 is a non-receptor tyrosine kinase with a molecular mass of approximately 120 kDa. It primarily localizes in the cytoplasm but can also translocate to the membrane and nucleus. This protein is ubiquitously expressed with notable presence in tissues like the brain liver and muscles. ACK1 plays a significant role in cellular signaling by phosphorylating various substrates impacting processes like cell proliferation migration and survival.
Biological function summary
The functions of ACK1 relate to signal transduction processes. It acts as an important part of the signaling complex involved in cell growth and differentiation. ACK1 interacts with several growth factor receptors and coordinates signal transduction events. These interactions allow ACK1 to participate in complex networks that regulate cellular shaping and organization making it important for normal cell functionality.
Pathways
ACK1 significantly influences cellular processes through the MAPK and PI3K pathways. These pathways are essential for cell survival proliferation and metabolism. ACK1 interacts with proteins such as ERK and AKT mediating downstream signaling that determines cellular responses to external stimuli. The kinase activity of ACK1 regulates various signaling cascades emphasizing its role in controlling metabolic and growth signals.
ACK1 shows relevance to cancer and neurodegenerative diseases. In cancer ACK1 overexpression associates with increased tumor growth and metastasis linked with proteins like HER2 in breast cancer. In neurodegenerative diseases abnormal ACK1 activity aligns with the progression of conditions like Alzheimer's disease where it interacts with tau proteins. Understanding ACK1's involvement in these diseases highlights its potential as a therapeutic target.