Abcam, ab74235, Anti-ATPAF2 antibody

Size: 100µg
Rabbit Polyclonal ATPAF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATPAF2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATPAF2. The exact immunogen used to generate this antibody is proprietary information.Q8N5M1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ATPAF2 protein also known as ATP synthase mitochondrial F1 complex assembly factor 2 plays a significant role in the mitochondrial ATP synthase assembly. It is approximately 33 kDa in size. This protein is expressed mainly in tissues with high-energy demands such as muscle and brain tissue. It aids in the proper assembly of the ATP synthase complex by supporting the integration of certain subunits which is vital for maintaining mitochondrial function and energy production.
Biological function summary
ATPAF2 assists in the proper assembly of proton-translocating ATP synthase complexes within mitochondria. It is not directly a part of the ATP synthase complex but acts as an assembly factor that facilitates the inclusion of F1 complex components. This participation is important for the generation of ATP via oxidative phosphorylation—a process pivotal for cellular energy management.
Pathways
ATPAF2 is essential for the efficient functioning of the oxidative phosphorylation pathway. This pathway is responsible for ATP production in cells via the mitochondrial electron transport chain. Within this pathway ATPAF2 relates closely with ATP5B and ATP5C1 proteins which form integral parts of the ATP synthase complex. These interactions help ensure that ATP synthase is constructed correctly enabling the efficient synthesis of ATP.
ATPAF2 mutations have been associated with mitochondrial diseases and neurodegenerative disorders. Mitochondrial abnormalities caused by improper ATP synthase assembly can lead to conditions like Leigh syndrome. Proteins like ATP5A1 and ATP5O which also participate in the biogenesis of ATP synthase can demonstrate interconnected malfunction when ATPAF2 is impaired contributing to the severity of these diseases.