Abcam, ab74556, Recombinant Hantavirus Dobrava, strain Slovenia, nucleocapsid protein

Size: 100µg
Recombinant Hantavirus Dobrava, strain Slovenia, nucleocapsid protein is a Dobrava-Belgrade orthohantavirus Full Length protein, expressed in Saccharomyces cerevisiae , with >95%, suitable for SDS-PAGE, ELISA, WB.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Saccharomyces cerevisiae,
Tags:Tag free,
Applications:WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q805Q9,
Animal free:No,
Carrier free:No,
Species:Dobrava-Belgrade orthohantavirus,
Reconstitution:Reconstitute at 0.5 mg/mL in PBS,
Storage buffer:Constituents: PBS

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hantavirus Dobrava strain Slovenia nucleocapsid protein also called N protein plays an important role in viral replication and assembly. This protein has a mass of approximately 50 kDa and is integral for encapsidating the viral RNA genome. It mainly expresses in infected host cells forming the basis of the ribonucleoprotein complex that is fundamental for the virus life cycle. The nucleocapsid protein serves as a scaffold for the construction of the viral capsid facilitating interactions with host cells' machinery.
Biological function summary
This nucleocapsid protein is involved in several critical processes for the virus. It forms part of a larger complex the ribonucleoprotein complex which includes the viral RNA and other key proteins. This complex serves as the template for replication and transcription of the viral genome ensuring the production of new viral particles. The N protein's ability to bind RNA and other nucleocapsid proteins makes it central to the regulation of these processes. Understanding its role provides insights into how hantaviruses hijack host cells for replication.
Pathways
The nucleocapsid protein from the Dobrava hantavirus strain participates in significant pathways related to viral replication and innate immune response evasion. It interacts with pathways involving the RIG-I-like receptor signaling helping the virus avoid detection by the host's immune system. Proteins such as RIG-I and MDA5 play a role here as they typically detect viral RNA but are often inhibited by this viral protein's functions allowing the virus to replicate undetected initially.
The nucleocapsid protein of the Dobrava hantavirus strain contributes to hemorrhagic fever with renal syndrome (HFRS) a severe disease caused by hantavirus infections. Its interaction with proteins such as interferon regulatory factors (IRFs) demonstrates its role in modulating immune responses exacerbating disease progression. Understanding the nucleocapsid's interactions and functions helps in identifying therapeutic targets to mitigate the effects of hantavirus-related diseases.