Abcam, ab75701, Anti-Granulocytes antibody [SPM250]

Size: 500µL
Mouse Monoclonal Granulocytes antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Granulocytes protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:SPM250,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab75701 reacts with early precursor and mature forms of human myeloid cells. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Product details:
ab75701 can be useful for the detection of myeloid leukemias and granulocytic sarcomas and also can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2 - 7.4Preservative: 0.09% Sodium azideConstituents: BSA, PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Granulocytes also known as polymorphonuclear leukocytes are immune cells essential for the body's defense mechanism against infections. These cells have a distinct lobed nucleus and are filled with granules that contain enzymes and other biologically active molecules. Weighing between 12-15 kilodaltons granulocytes are expressed in the bone marrow and circulate in the blood. They are classified into three types: neutrophils eosinophils and basophils each with unique granule content and functions.
Biological function summary
Granulocytes play a critical role in the innate immune response by engaging in phagocytosis degranulation and the release of cytokines. Neutrophils are part of the myeloid cell lineage and act as the body's first line of defense by engulfing and destroying pathogens. Eosinophils control parasitic infections and allergic responses while basophils release histamine in allergic reactions. Granulocytes work in coordination with other immune cells such as lymphocytes to form a complex immune system network.
Pathways
Granulocytes participate in pathways that involve inflammatory response and allergic reaction. Neutrophils are integral to the signaling pathway associated with the production of reactive oxygen species which helps eliminate pathogens. This process is linked with proteins like NADPH oxidase. Additionally other pathways involve eosinophils and basophils in modulating immune reactions through cytokine secretion which can also involve mediators like interleukin-5.
Granulocytes have been linked to conditions like chronic granulomatous disease and asthma. Chronic granulomatous disease results from a deficiency in NADPH oxidase impairing the granulocytes' ability to produce reactive oxygen species leading to recurrent infections. Asthma involves an overactive immune response with increased eosinophil activity contributing to airway inflammation. In these contexts granulocytes interact with other proteins including interleukins to modulate the disease progression.