Abcam, ab76013, Anti-TTF1/Nkx2-1 antibody [EP1584Y]

Size: 20µL / 100µL / 1mL
Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) is a rabbit monoclonal antibody detecting TTF1/Nkx2-1 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 170 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1584Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P and WB.
Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) was first used in a scientific publication in 2004 and has been cited over 175 times in peer reviewed journals. It's performance in IHC in mouse and human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) has 16 independent reviews from customers.
Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab76013) specifically detects TTF1/Nkx2-1 (UniProt ID: P43699; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1584Y -
ab216648
Antibody clone EP1584Y is also available pre-conjugated to a variety of labels for your convenience - Biotin (
ab314937
Top cited antibody in the market for this target with >250 citations. TTF1, also known as NKX2-1, is a crucial thyroid transcription factor involved in the development of the lung, thyroid and brain. It serves as a key marker in diagnosing lung cancer and is implicated in brain-lung-thyroid syndrome. Abnormal TTF1 expression is linked to various diseases, including non-small cell lung cancer and congenital hypothyroidism. Monitoring TTF1 levels can provide valuable insights into disease mechanisms and therapeutic targets.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TTF1 also known as Nkx2-1 is a transcription factor with a molecular mass of approximately 38 kDa. It plays a significant role in regulating gene expression. This target is mainly expressed in tissues such as the thyroid lung and the brain. In the thyroid it activates genes associated with thyroid hormone synthesis while in the lung it regulates genes important for lung development and function. It is also referred to by other alternate names such as anti-TTF1 and NKX2.1.
Biological function summary
TTF1/Nkx2-1 is essential for organ-specific development and maintenance. It is a critical component of transcriptional complexes that modulate the expression of genes during the development of the thyroid and lung tissues. TTF1/Nkx2-1 impacts cellular processes by controlling genes involved in differentiation. Its regulatory functions play an important role in dictating cell fate decisions and maintaining organ identity.
Pathways
The activity of TTF1/Nkx2-1 integrates into the TGF-beta signaling and Wnt pathways. These pathways are imperative for cellular proliferation and differentiation. TTF1/Nkx2-1 possesses interactions with proteins like SMADs within the TGF-beta pathway influencing cellular responses. Similarly it interacts with beta-catenin in the Wnt pathway contributing to the modulation of gene expression relevant to developmental processes.
TTF1/Nkx2-1 has been associated with conditions like lung cancer and congenital hypothyroidism. Altered expression or mutations in this transcription factor can lead to these disorders. For instance in lung cancer the aberrant expression of TTF1/Nkx2-1 may involve proteins such as EGFR which contributes to tumorigenesis. In congenital hypothyroidism disruptions in TTF1/Nkx2-1 activity can affect thyroid hormone synthesis-related proteins impacting metabolic processes.