Abcam, ab76126, Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker

Size: 20µL / 100µL / 1mL
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker (ab76126) is a rabbit monoclonal antibody detecting Cytokeratin 20 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2009
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1622Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen of this antibody is 73% homolog with Mouse-Cytokeratin 20. This antibody gives positive results for mouse samples in Western Blot only. Therefore we do not recommend this antibody for mouse samples and do not cover mouse with our Abpromise guarantee.

Product details:
What is this antibody validated in?
Anti-Cytokeratin 20 antibody [EPR1622Y] - Cytoskeleton Marker (ab76126) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Cytokeratin 20?
Anti-Cytokeratin 20 [EPR1622Y] - Cytoskeleton Marker (ab76126) specifically detects a band for Cytokeratin 20 (UniProt: P35900) at a molecular weight of 48kDa.
Trusted by the scientific community
Anti-Cytokeratin 20 [EPR1622Y] - Cytoskeleton Marker (ab76126) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Cytokeratin 20 [EPR1622Y] - Cytoskeleton Marker (ab76126) has over 15 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytokeratin 20 often known as CK20 KRT20 or keratin 20 is a type of intermediate filament protein that is highly specific to epithelial cells. This cytoskeletal protein has a molecular weight of approximately 46.5 kDa. Cytokeratin 20 is primarily found in the gastric and intestinal epithelium urothelium and Merkel cells. Its function as part of the cytoskeleton provides structural integrity and strength to cells maintaining their shape and resilience against mechanical stress.
Biological function summary
Cytokeratin 20 is essential in cellular differentiation and maintenance within epithelial tissues. It plays an important role in the structural framework of epithelial cells by integrating into the cytoskeletal network which includes other keratins. Typically cytokeratin 20 forms heterodimers with keratin 8 contributing to the assembly of intermediate filaments. These interactions maintain cell stability and are involved in cellular processes such as migration and proliferation.
Pathways
Cytokeratin 20 participates in the framework of intermediate filament signaling influencing cell motility and secretion in epithelial tissues. It contributes mainly to the keratinization pathway which is critical in forming a protective barrier in epithelial cells. Additionally it is related to cell adhesion and signalling pathways that are important for tissue integrity. Connections with keratin 8 and other cytoskeletal proteins facilitate signal transduction processes necessary for epithelial homeostasis and response to injury.
Cytokeratin 20 serves as a valuable marker for identifying specific cancer types particularly colorectal adenocarcinomas and Merkel cell carcinoma. The presence of cytokeratin 20 is often used in immunohistochemistry for cancer diagnosis and prognosis. Its expression pattern is notably distinct helping differentiate between various adenocarcinomas. In colorectal cancer it shows a unique interaction with proteins like keratin 8 supporting the tumor's structural environment and influencing tumorigenesis and metastasis processes.