Abcam, ab76129, Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1491Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.This antibody also detects various PKC phosphorylated isoforms.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase C zeta and iota (PKCζ and PKCι) also known as PRKCZ and PRKCI are important members of the atypical PKC subgroup within the larger PKC family. PKCζ and PKCι facilitate signal transduction through phosphorylation a process essential for modifying protein activity. These proteins have an approximate mass of 67 kDa. They are found in various tissues with expression in the brain lung and kidney being notably high. Both PKCζ and PKCι participate in cell processes by acting through the phosphorylation of specific substrate proteins.
Biological function summary
Atypical PKCs such as PKCζ and PKCι regulate numerous cellular activities including cell proliferation survival and differentiation. These proteins often exist as part of larger signaling complexes. For example PKCζ functions within a complex involving Par-6 and Par-3 playing an important role in maintaining cell polarity. PKCι similarly contributes to cellular processes essential for growth and development by interacting with several proteins involved in signaling cascades.
Pathways
PKCζ and PKCι integrate into signaling networks like the PI3K/Akt pathway which is critical for controlling cell growth and survival. These atypical PKCs do not need calcium or diacylglycerol for activation unlike other PKCs. They interact with proteins such as p62 and mTOR influencing cell metabolism and apoptosis. Through these interactions PKCζ and PKCι help modulate important cellular responses to extracellular signals.
PKCζ and PKCι have established connections with conditions like cancer and metabolic syndrome. Dysregulation of PKCζ can contribute to tumorigenesis by affecting processes like apoptosis and cell cycle control. PKCι has links to lung cancer and is implicated in improper cell cycle regulation. During these conditions PKCζ and PKCι often show abnormal interactions with proteins such as Akt and mTOR highlighting their role in pathological processes affecting cell growth and metabolism.